The automacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. Alfa laval mapx207 separator technical specifications for repair. The automacs pro separator is equipped with reusable automacs columns. The macs minisampler, attached to the automacs pro separator, can hold one of three different macs cooling tube racks containing up to six samples. Check the list of manual separators below and choose the one that fits the column type, cell number, sample number, and sample size you are working with. The instrument status can be viewed from a distance, facilitated by illumination of buffer bottles by lightemitting diodes. Magnetic cell separation was carried out using the automacs separator miltenyi biotec by referring to the automacs user manual and applying the separation program depletes. The components of an automacs pro separator starter kit. Content 5 content 5 content chapter 1 15 1 important information 15 1. A xerxes oilwater separator is a highquality system for removing oil from water.
The following warnings and cautions are provided to help you prevent injury to yourself or damage to the instrument. After priming, the instrument will automatically display the status menu. The automacs pro separator is a compact, high performance immunomagnetic cell separation instrument with fully automated functions ideal for the processing of multiple samples with minimal handson manipulation. Watch how cell isolation on the automacs pro separator works with the automacs pro separator, cells from whole blood or bone marrow can be isolated with minimal handson time.
If you want to use it inside the hood, do not forget to. This chapter describes the safety instructions and site requirements for your automacs pro separator. If the separator has been delivered and installed by alfa laval as a part of a processing system, this manual should be. Employing the macs technology, the automacs separator is designed for positive selection as well as depletion of magnetically labeled cells. The miltenyi biotec automacs pro works with a variety of cell separation reagents used for quick isolation of. Stanford automacs pro user guide 072211 starting up 1. Persons who have not read and understood the instruction manual of the separator. The automacs pro separator and the macs minisampler are designed to operate safely after installation and when used by trained personnel according to general safety practices and the instructions set forth in this user manual. Columns do not have a top or bottom and do not require special orientation in the column holder.
Macsmiltenyibiotecde automacs pro separator user manual 3 read the user manual before using the instrument before using the instrument, read chapter important safety information and all other information contained in this user manual, including all. This chapter describes the safety instructions and site. Cells were then washed and resuspended in 1 ml of macs buffer. Keep this user manual and any other safety and operating instructions provided with the instrument in a safe place that is accessible to all users for reference. Magneticlabeling, separation of cells, and system rinsing are completely. Keep this user manual in a safe place, accessible for anyone using the. Automacs pro separator magnetic cell sorter youtube. Buffers used for operating the automacs separator or the automacs pro separator should have a temperature of. If the separator has been delivered and installed by alfa laval as. The automacs pro separator automatically records the date. Switch on the automacs pro separator the instrument will automatically initialize. Please read the user manual of this instrument carefully and pay attention to all warnings and precautions.
Magnetic separation with the automacs separator or the automacs pro separator refer to the respective user manual for instructions on how to use the automacs separator or the automacs pro separator. Consult the user manual before operating this instrument for the first time. Check that all bottles are filled with the appropriate solutions. Persons who have not read and understood these basic safety precautions. Persons who have not been briefed on proper and correct operation. Keep this user manual in a safe place, accessible for anyone using the automacs pro separator. Jul 18, 2016 automacs pro separator magnetic cell sorter available from the lab world group. Protocol overview we use miltenyi beads to capture the cell surface markers of interest. Unless otherwise specifically indicated, miltenyi biotec products and services are for research use only and not for therapeutic or diagnostic use. Our innovative tools support research at every level, from basic research to. Place tube containing the magnetically labeled cells in the automacs separator.
The macs products are sold by miltenyi biotec online catalogue at. The automacs pro separator combines all the benefits of the proven automacs technology with novel features such as automated magnetic labeling and sensorcontrolled processing of multiple samples. Lab, and analytical equipment from leading ma biotech. Maximal care has been taken by miltenyi biotec in the preparation of this user manual. Morrison 9102014 page 23 the automacs pro separator is a benchtop instrument for highspeed automated magnetic cell sorting of multiple samples under standardized conditions.
Magnetic cell separation was carried out using the automacs separator miltenyi biotec referring to the automacs user manual applying the separation program posseld2. Magnetic separation with the automacs pro separator or the automacs separator refer to the respective user manual for instructions on how to use the automacs pro separator or the automacs separator. Enrichment of cell subpopulations applying automated macs. Alfa laval separators manuals and spare parts catalogs in. Depletion with the automacs pro separator or the automacs separator refer to the respective user manual for instructions on how to use the automacs pro separator or the automacs separator. After each cell separation, a thorough washing procedure rinses the columns of the automacs pro separator. Manual cell separators for fast and gentle cell isolation. If in doubt contact your local sales representative or miltenyi biotec automacs pro specialist. By combining an exceptionally compact design, a simple, userfriendly interface and the fast and easy cell separation protocols youve come to expect from stemcell technologies, roboseps fits easily into the workflow of any lab that needs the multisample processing capacity, speed, reliability and convenience of automated cell isolation. Choose a separation program according to the recommendations in the automacs user manual. Buffers used for operating the automacs pro separator or the manual. Custom mode users and administrators can perform manual calibration. The cell separation is programmed using the touchscreen and intuitive screen menus.
Automacs pro separator magnetic cell sorter available from the lab world group. Empty the waste bottle and add 100ml bleach to the bottom. Russian language 401210 mapx 309 alfa laval mapx309 separator. Automated cell isolation automacs pro separator usa. Retain this user manual and any other safety and operating instructions provided with the instrument in a place accessible to all users for future. The macs minisampler connects to the automacs pro separator and thus represents a part of the cell separation device. A utomacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. Alfa laval separators manuals and spare parts catalogs in pdf. Automacs user guide the automacs allows biomagnetic cell sorting of cells labelled with macs microbeads. The automacs pro separator is a benchtop automated magnetic cell sorter for the processing of multiple samples. When using the program posselwb, the whole blood sample will be diluted 3fold the starting volume. To view the current software version, from the software menu select.
Magnetic separation with automacs separator refer to the automacs user manual for instructions on how to use the automacs separator. Miltenyi automacs cell separator macs, e includes manual, software disc, and all pictured accessories, sn. Common and widely accepted general safety precautions must be taken when operating the machinery. Ap studio series sl automatic products studio series sl. Miltenyi automacs cell separator macs, e includes manual. Designed for the selection of target cells in a fully automated, walkaway fashion. Buffers used for operating the automacs pro separator or the automacs separator should have a temperature of.
The miltenyi biotec automacs pro is a powerful automated cell separator used to isolate cells from whole blood, or bone marrow. For detailed information on the separator and column type recommended for a specific reagent, please refer to the data sheet provided with the particular macs cell separation reagent. Positive selection labeling of target cells frequency of target cells. Place tube containing magnetically labeled cells in automacs. The automacs allows biomagnetic cell sorting of cells labelled with macs. Magneticlabeling, separation of cells, and system rinsing are completely automated. Pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. Miltenyi biotec is a global provider of products and services that advance biomedical research and cellular therapy. The automacs pro separator is designed for safe use when installed correctly, operated by trained personel operated in accordance with general safety practices and the instructions in this user manual. Magnetic separation with the automacs separator refer to the automacs user manual for instructions on how to use the automacs separator. Warnings and precautions the guidelines in this section explain the potential risks associated with the. The automacs pro separator combines all the benefits of the proven automacs technology with. Manual and the installation manual should be interpreted so as.
Eys separator eys 01 g user manual for eys 01 g manure separator 3 2 general safety instructions for eys 01 g check the operational safety of the machine before every startup 1. For a standard separation, choose following separation programs. Technology, the automacs pro separator is designed for cell isolation in a fully automated, walkaway fashion. Manual cell separators for fast and gentle cell isolation usa. Cells are incubated at the specified dilution in the bead kit for 15 min at 4 degrees celcius, then washed with running buffer, spun down at 1200 rpm for 10 min, and resuspended in 2 ml of running buffer in 15 ml conical tubes.
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